Player FM - Internet Radio Done Right
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Kandungan disediakan oleh Taylor & Francis Group and Francis Group. Semua kandungan podcast termasuk episod, grafik dan perihalan podcast dimuat naik dan disediakan terus oleh Taylor & Francis Group and Francis Group atau rakan kongsi platform podcast mereka. Jika anda percaya seseorang menggunakan karya berhak cipta anda tanpa kebenaran anda, anda boleh mengikuti proses yang digariskan di sini https://ms.player.fm/legal.
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You Can’t Make This Up
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At the dawn of the social media era, Belle Gibson became a pioneering wellness influencer - telling the world how she beat cancer with an alternative diet. Her bestselling cookbook and online app provided her success, respect, and a connection to the cancer-battling influencer she admired the most. But a curious journalist with a sick wife began asking questions that even those closest to Belle began to wonder. Was the online star faking her cancer and fooling the world? Kaitlyn Dever stars in the Netflix hit series Apple Cider Vinegar . Inspired by true events, the dramatized story follows Belle’s journey from self-styled wellness thought leader to disgraced con artist. It also explores themes of hope and acceptance - and how far we’ll go to maintain it. In this episode of You Can't Make This Up, host Rebecca Lavoie interviews executive producer Samantha Strauss. SPOILER ALERT! If you haven't watched Apple Cider Vinegar yet, make sure to add it to your watch-list before listening on. Listen to more from Netflix Podcasts .…
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Kandungan disediakan oleh Taylor & Francis Group and Francis Group. Semua kandungan podcast termasuk episod, grafik dan perihalan podcast dimuat naik dan disediakan terus oleh Taylor & Francis Group and Francis Group atau rakan kongsi platform podcast mereka. Jika anda percaya seseorang menggunakan karya berhak cipta anda tanpa kebenaran anda, anda boleh mengikuti proses yang digariskan di sini https://ms.player.fm/legal.
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288 episod
Tandakan semua sebagai (belum) dimainkan
Manage series 1258384
Kandungan disediakan oleh Taylor & Francis Group and Francis Group. Semua kandungan podcast termasuk episod, grafik dan perihalan podcast dimuat naik dan disediakan terus oleh Taylor & Francis Group and Francis Group atau rakan kongsi platform podcast mereka. Jika anda percaya seseorang menggunakan karya berhak cipta anda tanpa kebenaran anda, anda boleh mengikuti proses yang digariskan di sini https://ms.player.fm/legal.
Podcast by Taylor & Francis Group
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288 episod
Alle Folgen
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1 Cell therapies for autoimmune diseases: a podcast with Lindsay Davies 13:17
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This International Women’s Day, we’re joined by Lindsay Davies, Chief Scientific Officer at NextCell Pharma (Stockholm, Sweden), to discuss the evolving role of cell therapies in treating autoimmune diseases. In this podcast, Davies breaks down the science behind NextCell’s mesenchymal stromal cell therapy for type 1 diabetes and explores how these cells could be applied to other autoimmune conditions. She emphasizes the importance of tailoring cell therapies based on disease type, stage and patient profile to achieve the best outcomes. The conversation also highlights how growing evidence of sex-related differences will could increasingly shape decisions in the development and testing of cell therapies.…
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1 The use of GenAI in bioanalysis: an interview with Priyata Kalra 28:30
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During the American Association of Pharmaceutical Scientists (AAPS) PharmSci 360 2024 conference, Bioanalysis Zone caught up with industry experts across numerous disciplines to discuss their involvement with AAPS and their research. In this interview, we spoke to Priyata Kalra about her talk on the use of GenAI within biomedicine.…
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1 The need for HRMS for quantitation of ADCs vs. QQQ: an interview with Megan Cooley 21:35
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In this episode, we sit down with Megan Cooley, Associate Director of Bioanalytical Services at ICON (KS, USA), to explore the cutting-edge world of antibody-drug conjugate (ADC) quantitation using high-resolution mass spectrometry (HRMS). Megan delves into the capabilities of Triple Quadrupole and Time-of-Flight mass spectrometry, offering her expert insights into whether HRMS is essential for ADC PK studies. We also tackle the challenges and limitations of HRMS in ADC quantitation and discuss the potential for AI to create more specific and stable ADCs. Finally, Megan highlights ICON’s extensive experience with large molecule applications and their innovative approach to supporting complex LC–MS analyses.…
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1 How can medical device regulation catch up with innovation? 12:36
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In this interview, we discuss the potential of AI in increasing healthcare system efficiency, how medical devices are making procedures less invasive and what those working in the field of medical devices can do to help regulation catch up with innovation.
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1 The new frontier: assessing efficacy and safety of CGTs using biomarkers 24:21
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In the sixth episode of The new frontier podcast, we're joined by Catherine Vrentas, Life Sciences Lead at Booz Allen Hamilton (VA, USA). Cathy explains how biomarkers can be used to predict the therapeutic outcome and efficacy of CGTs and the biomarkers in particular that have shown strong predictive value. We delve into the common challenges of working with biomarkers in CGTs and how biomarker kits can be adapted for CGT work.…
Carlon Fitzpatrick AI Summit by Taylor & Francis Group
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1 Challenges and solutions in monitoring assay performance for vaccine development 18:26
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Challenges and solutions in monitoring assay performance for vaccine development: an interview with Atul Chaudhari
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1 ADA detection and characterization for immunogenicity testing: insights from ICON's Lindsay Denhoff 11:13
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We caught up with Principal Scientist Lindsay Denhoff (ICON; Dublin, Ireland) to discuss immunogenicity considerations for therapeutic proteins and the current analytical methodologies for detecting and characterizing anti-drug antibodies (ADAs). Lindsay shares some industry best practices for achieving high-quality ADA assays, as well as some of the advantages and limitations of singlicate ADA analysis compared with alternative techniques. We finish the interview with a look to the future where Lindsay discusses the emerging trends she's spotted in the field of immunogenicity and offers her predictions for how they may shape the future of therapeutic protein development.…
In this Tech Blast episode supported by Sartorius (Göttingen, Germany), we speak with David Apiyo – Manager of Applications Development at Sartorius (MN, USA) – about epitope binning, why it's performed and the best ways to conduct it. Plus, learn more about Sartorius' biolayer interferometry platforms and their application in epitope binning.…
In this interview, we spoke to John Chappell, Senior Global Scientific Support Manager at Gyros Protein Technologies (Uppsala, Sweden) about his experience working with the Gyrolab platform for oligonucleotide assays. John discusses the advantages of using Gyrolab for oligo quantification in biological fluids and shares his thoughts on the next steps for these assays.…
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1 The new frontier: qPCR vs ddPCR for cell and gene therapies 15:41
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In the fifth episode of The New Frontier podcast, we're joined by Paul Byrne, Senior Director of Genomics from ProtaGene (MA, USA). Paul describes his experience working with qPCR vs digital and digital droplet PCR assays and advises on their pros and cons, as well as when it's best to use each. We discuss how these assays can be optimized for cell and gene therapy targets and get a peek into the tips and tricks he's picked up over his 30 years of experience.…
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1 The new frontier: using LC–MS/MS for quantitative assays to support gene therapies 13:33
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In this episode of The New Frontier podcast, we're joined by Daniel Schulz-Jander, Senior Director of Mass spectrometry Bioanalysis at QPS Netherlands (Groningen). Daniel explains how he uses immune-precipitation and immunoaffinity LC–MS techniques for his work relating to macromolecular pharmaceuticals like gene therapies, as well as their benefits and challenges. Daniel also covers bottom-up, middle-down and top-down approaches and their suitability for his work in clinical support versus research and discovery.…
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1 Breaking down barriers for women in tech: an interview with Tina Marshall 16:15
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Breaking down barriers for women in tech: an interview with Tina Marshall by Taylor & Francis Group
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1 Uncovering the link between cancer disparities and climate change 14:35
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What disparities exist in cancer screening and how does this impact patient outcomes? Listen to our latest podcast episode with Kilan Ashad-Bishop (American Cancer Society) to find out about the intersection between science, health, and society.
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1 AI-driven early disease detection: an interview with Jim Kean 28:19
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AI-driven early disease detection: an interview with Jim Kean by Taylor & Francis Group
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1 The new frontier: immunogenicity testing for cell and gene therapies with Johanna Mora 11:01
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In this episode, we spoke to Senior Director Johanna Mora about her experience with immunogenicity testing for cell and gene therapies (CGTs) and how she prepared for the expansion of BMS's portfolio to include CGTs. We discuss the importance of having diverse teams with a broad range of experiences to solve complex problems, as well as the role of artificial intelligence in improving our efficiency and spotting patterns in the data.…
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1 The new frontier: regulatory implications for cell and gene therapies with Kimberley Buytaert-Hoefen 18:01
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In the first episode of this series, we spoke to Executive Director and Global Head of Regulatory Services Kimberley Buytaert-Hoefen about her experience with cell and gene therapy (CGT) approvals and the barriers to getting these important therapeutics to the patient. Kim advises early regulatory interaction and collaboration to improve the success rate of applications and emphasizes the importance of global harmonization of regulatory standards. We discuss key barriers to CGT approvals including manufacturing and immunogenicity and Kim voices her excitement for the newly approved CRISPR-Cas9 technology to treat sickle cell disease.…
AI and the UK Biobank: an interview with Mahesh Pancholi
In our new podcast series, we're joined by a Bioanalysis author to review their publication's biggest challenges, wider implications and next steps. In the final episode of series one, we're joined by Hetal Sarvaiya and Yuting Wang from AbbVie (MA, USA), who talk about the challenges of having an international team working on their perspective article and the inspiration behind their work. The pair talk passionately about their paper and how the unique nature of AbbVie's global team has helped shape their work.…
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1 Virtual reality therapies for mental health: Marta Matamala-Gomez 19:35
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Virtual reality therapies for mental health: Marta Matamala-Gomez
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1 An interview with Namshik Han: predicting protein structure with AI 15:45
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An interview with Namshik Han: predicting protein structure with AI
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1 Tech Blast | Surface plasmon resonance 12:55
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In this Tech Blast episode supported by Sartorius, we speak with Derrick Christopher Campos, Product Manager at Sartorius (TX, USA), about Surface Plasmon Resonance (SPR). This tried-and-true technique allows researchers to do label-free protein characterization; in this podcast, Derrick discusses how Sartorius improves upon multi-cycle kinetics with their OneStep® gradient injection.…
In our new podcast series, we're joined by a Bioanalysis author to review their publication's biggest challenges, wider implications and next steps. In episode four, we're joined by Senior Scientist and Technical Specialist Ashley Phillips. Ashley's publication assessed the performance of next-generation trypsins for rapid protein LC-MS bioanalysis, effectively reducing a two-day process into just one day's work. As the paper's lead author, Ashley outlines the experimental design, the challenges associated with data storage and how he and the team are now applying the best-performing trypsin in their workflows.…
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1 Beyond the Abstract: an interview with Camilla Linder & Miguel Barroso 11:09
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In our new podcast series, we're joined by a Bioanalysis author to review their publication's biggest challenges, wider implications and next steps. In episode three, Digital Editor Ellen Williams is joined by Clinical Instructor and Education Coordinator Camilla Linder and Biomedical Laboratory Scientist Miguel Gambell Barroso. The collaborative publication details an inventive method for determining hematocrit in dried blood spots using image analysis to avoid sample damage. Camilla and Miguel discuss the origins of the work, the challenges involved and even hint at a unique use for their method.…
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1 An interview with Walt Szablowski: how should healthcare systems handle cybersecurity breaches? 10:10
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An interview with Walt Szablowski: how should healthcare systems handle cybersecurity breaches? by Taylor & Francis Group
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1 Talking Techniques | CAR-T and CAR-NK cell therapies 27:35
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In this episode of Talking Techniques, Ritwika Biswas, Field Scientist at Sino Biological US Inc. (PA, USA), walks us through the chimeric antigen receptor (CAR) development process and how T and natural killer (NK) cells can be utilized to carry these modular synthetic molecules. Ritwika also addresses the safety and efficacy of these cell therapies as well as the ethical considerations around them.…
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1 Talking Techniques | CAR-T and CAR-NK cell therapies 27:35
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In this episode of Talking Techniques, Ritwika Biswas, Field Scientist at Sino Biological US Inc. (PA, USA), walks us through the chimeric antigen receptor (CAR) development process and how T and natural killer (NK) cells can be utilized to carry these modular synthetic molecules. Ritwika also addresses the safety and efficacy of these cell therapies as well as the ethical considerations around them.…
AI Summit 2023: an interview with Phalgun Lolur by Taylor & Francis Group
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1 Brain-computer interfaces and neuroethics with Uthman Ali 16:53
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Brain-computer interfaces and neuroethics with Uthman Ali by Taylor & Francis Group
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1 Brain-computer interfaces and neuroethics with Uthman Ali 16:53
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Brain-computer interfaces and neuroethics with Uthman Ali by Taylor & Francis Group
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1 Tech Blast | Preparing 3D cell cultures 11:02
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In this Tech Blast episode, we explore preparing 3D cell cultures with Sartorius’ (Goettingen, Germany) Sandra Söderholm, Application Development Specialist, and Maria Voutilainen, Product Manager. Sandra and Maria give us an introduction to 3D cell cultures and how to prepare them, and then delve into a foundational technique that is often overlooked: pipetting. We discuss pipetting techniques, common mistakes and their pipetting dos and don’ts. Contents: Introduction: 00:00–01:30 An introduction to 3D cell cultures: 01:30–02:48 Preparing 3D cell cultures: 02:48–03:25 Don’t overlook your pipetting technique: 03:25–05:45 Common pipetting mistakes and how to overcome them: 05:45–08:30 Reproducibility of 3D cell cultures: 08:30–10:30…
In our new podcast series, we're joined by a Bioanalysis author to review their publication's biggest challenges, wider implications and next steps. In episode two, Ellen is joined by Research Assistant Gaudry Troché to discuss conventional sampling and microsampling for the comparison of pharmacokinetic study profiles of insulin. Gaudry discusses life as an early career scientist, his plans for the future and his poster presentation at the Health Benefits and Leadership conference in Las Vegas.…
In this episode of Talking Techniques, Ritwika Biswas, Field Scientist at Sino Biological US Inc., walks us through the chimeric antigen receptor (CAR) development process and how T and natural killer (NK) cells can be utilized to carry these modular synthetic molecules. Ritwika also addresses the safety and efficacy of these cell therapies as well as the ethical considerations around them.…
Lance - Interview - FINAL by Taylor & Francis Group
Luciano Floridi Interview Final by Taylor & Francis Group
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1 Mitochondria, the immune system and cancer: discovering new insights with spatial technologies 24:28
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In this episode, supported by Fortis Life Sciences, we delve into the relationship between mitochondria, inflammation and cancer, discussing the new techniques that are bearing fruit in this field, such as spatial analysis. Our expert insight for this episode comes from Phillip West, Principle Investigator of the West lab at Texas A&M Medicine (TX, USA). Philip explains the role mitochondria can play in cancer and heart disease, reveals some of his most exciting discoveries of late and provides technical tips for investigating this field. Listen on to discover how his use of spatial techniques has helped uncover mechanisms linking mitochondrial damage to the stifling of the immune system in the tumor microenvironment and the latest breakthroughs at the intersection of mitochondria and cancer. Contents: Introduction: 00:00-01:30The innate immune system, in inflammation and disease: 01:30-04:20The role of mitochondria in innate immunity: 04:20-07:00Categorizing DAMPS and their role in cardiovascular disease: 07:00-08:40Mitochondria and cancer: 08:40-11:55Techniques for the investigation of mitochondria: 11:55-15:20Best practice techniques for spatial studies: 15:20-17:35Discoveries made using spatial approaches to mitochondrial investigations: 17:35-19:55The latest developments in the intersection of mitochondria, cancer and inflammation: 19:55-22:20What is one thing you would ask for to improve your understanding of this field? 22:20-24:20…
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1 Intelligent health systems: an interview with Mazin Gadir 17:28
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Intelligent health systems: an interview with Mazin Gadir by Taylor & Francis Group
In this Tech Blast episode supported by CN Bio (Cambridge, UK), their Lead Scientist Emily Richardson and Principal Bioengineer Dharaminder Singh talk us through organ-on-a-chip (OOC) technologies. From what they are and how they work to what kind of data they provide and their future applications, Emily and Dharaminder provide all the information you need to understand the basics of OOC.…
In our new podcast series, we're joined by a Bioanalysis author to review the publication's biggest challenges, wider implications and next steps. In this first episode, we're joined by early career Researcher Anthony Breton to discuss volumetric absorptive microsampling (VAMS®) as an alternative to venepuncture for the assessment of essential and toxic elements.…
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1 Mixed reality technologies in medical and surgical applications: an interview with Taylor Kantor 27:44
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Mixed reality technologies in medical and surgical applications: an interview with Taylor Kantor
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1 Long-read sequencing for the metagenomic analysis of microbiomes 22:18
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In this episode, sponsored by Zymo Research, we explore the microbiome and how long-read sequencing techniques are changing our approach to the field and the exciting discoveries that they have led to. To do this we speak to Kris Locken, Molecular Biologist in the Research & Development department of Zymo Research (CA, USA), and Jeremy Wilkinson, Global Marketing Specialist for Microbial Genomics at PacBio (CA, USA). Find out how microbes compare to nuts and what this means for the challenges of metagenomic sample preparation, how long reads can stack up vs short reads for metagenome assembly and much more, all in this latest episode of Talking Techniques Contents:Intro: 00:00-01:55 What is metagenomics and why is it important for microbial studies? 01:55-03:55 How has long-read sequencing impacted metagenomics? 03:55-06:05 Long-read vs short-read sequencing workflows: 06:05-07:15 Addressing barriers to long-read sequencing: 07:15-08:45 Sample preparation for long-read sequencing: 08:45-12:20 Development of long-read sequencing to improve accuracy and capabilities: 12:20-14:40 Best practice for assembly and analysis: 14:40-17:40 Exciting examples of the benefits of long-read sequencing: 17:40-20:00 What would you wish for to improve the ability long-read sequencing in microbiomics: 20:00-21:40…
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1 Tech Blast | Gene expression analysis 12:32
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In this Tech Blast episode supported by Thermo Fisher Scientific (MA, USA), Associate Product Manager Jon Flasher talks us through gene expression analysis, some of its challenges and the tools available to streamline the process. Listen to learn more about how Thermo's TaqMan Gene Expression Assays can be used to address research questions around cancer, biomarker identification, hereditary diseases, neurological diseases and the body's response to pathogens like COVID-19. Products mentioned in this podcast are for research use only, not for use in diagnostics.…
In this episode of Editor’s Picks, hear about how researchers have taken biotechnology to a whole new level, moving individual cells with sound waves, and geckos are more socially intelligent than we thought….
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1 The power of intentional mentoring: an interview with Heather Beasley 19:04
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Hello and welcome to the latest episode of OCTalks, the podcast series from Oncology Central. I am Jade Parker, Senior Editor of Oncology Central. Today I am joined by Heather Beasley who is a post-doctoral scholar at Vanderbilt University (TN, USA). Heather’s research focuses on calcium signaling in breast cancer, as well as the dysregulation of bone metabolism due to comorbidities such as hypercalcemia.…
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1 Open neuroscience and the meaning of FAIR 37:53
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This episode, our second recorded at Neuroscience 2022 (13-19th April 2022; San Diego, CA, USA), delves into the importance of open data in neuroscience and the FAIR guidelines, which encourage researchers to make their data Findable, Accessible, Interoperable and Reusable. Sharing her considerable expertise in this area is one of the authors of the FAIR guidelines, Maryann Martone, who provides some key examples of the successes that open data practices have delivered so far and cautionary tales for how current practices are damaging the field. Listen on to find out how to implement open data practices, how they can help your lab and why Maryann sees it as our responsibility to resolve! Contents: Introduction: 00:00-02:00Introducing open neuroscience and the meaning of FAIR 02:00-03:00Exposing outdated paradigms in science: academic targets, publications, reproducibility and data accessibility: 03:00-06.30Incentivizing open data and reassigning value restructuring academia: 06:30-08.40The impact of FAIR within labs: 08:40-09:50Challenges of establishing and barriers to Open and FAIR neuroscience 09:50-12:00The reception of these guidelines in the field: 12:00-16:50Examples of the impact of open data in the spinal cord injury community: 16:50-18:10Marryann’s experience of enacting changes early in her career: 18:10-21:20Judging value in scientific research and understanding your purpose: 21:20-24:20The importance of investment: 24:20-26:30The impact of industry on lab data: 26:30-27:50Practical tips for addressing your lab data: 27:50-31:10Key tips for preparing data for an open-source repository 31:10-33.15The FAIR data principles explained: 33.15-37:50…
In this Tech Blast episode, we discuss lab automation with Gregory Lee, Automation Manager at Zymo Research (CA, USA). We explore the types of tasks that can be automated, Greg’s three Ts to consider when thinking about automation ¬– technical, throughput and turnaround time – and his top tips for implementing automation. Greg also shares common pitfalls labs fall into when attempting to deploy automation, reminding us to never forget about the box. Contents: • Introduction: 00:00–00:40 • What does automation look like in a lab setting? 00:40–01:40 • What tasks can be automated? 01:40–02:30 • Greg’s three Ts: 2:30–03:50 • The benefits of automation: 03:50–05:15 • Greg’s top tips for implementing automation: 05:15–07:00 • Common pitfalls – it’s not all about the instrument: 07:00–08:10 • Think outside the box, but don’t forget it exists: 08:10–09:05 • Is automation our future? 09:05–10:45…
On this month’s episode of Editor’s Picks, we’re celebrating the 40th birthday of PCR, saving the northern white rhinoceros from the brink of extinction and connecting the dots between HPV, the vaginal microbiome and race.
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1 Welcome to the jungle: sequencing for conservation 43:22
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In this episode of Talking Techniques, we head to the Ecuadorian rainforest to chat about the use of sequencing in conservation with Zane Libke, a field researcher based at Sumak Kawsay In Situ (Pastaza, Ecuador). Zane discusses how he turned his fascination with nature into a career, his current work using nanopore sequencing technologies to find undescribed species and helping train future field researchers. We also explore the benefits of fighting biodiversity in a more local-centric way and using research to protect the area from exploitative companies. Contents:Intro: 00:00–01:35Zane’s work at Sumak Kawsay In Situ: 01:35–05:05Turning a fascination with nature into a career: 05:05–08:20The importance of sequencing for conservation: 08:20–12:25Technologies being used to sequence in the field: 12:25–14:15Portable nanopore sequencing with the MinION: 13:15–15:35Exporting samples means exporting opportunity: 15:35–18:40The sequencing workflow: 17:40–24:50Data processing: 24:50–27:45Using these technologies to find undescribed species: 27:45–32:20Fighting biodiversity loss in a more local-centric way: 32:20–34:55Using research to protect the area from exploitation: 34:55–38:50What the future holds: 38:50–43:22…
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1 Neuropixels: big data heaven or burying the lead in averages? 19:30
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In this special episode of Talking techniques, one of two recorded at Neuroscience 2022, we speak to Tim Harris (Howard Hughes Medical Institute, VA, USA). Tim is the creator of Neuropixels, neural recording probes inserted into the brains of animal models, which allow researchers to collect signals from hundreds of individual neurons in different brain regions simultaneously over extended periods of time. Here, he shares the story behind his creation and details how they have changed the data collection landscape in Neuroscience. Tim also considers every inventor’s greatest concern: has his invention led to purely positive outcomes for the field? By enabling the collection of huge datasets have Neuropixels led to studies that bury findings in vast swathes of data or have they enabled researchers to collect enough information to discover the emphatic truth? Listen now to find out Tim’s opinions on these contentious debates in neuroscience and get key tips for utilizing neuropixels! Contents:Introduction: 00:00-03:00Tim's symposium on the capabilities of Neuropixels: 03:00-04.00Key takeaways from the symposium: 04:00-07:45The story of the development of neuropixels: 07:45-13:50Best practice tips for utilizing neuropixels: 13:50-15:15Challenges of utilizing neuropixels: 15:15-15:50Addressing the challenge of big data and sharing uncertainty: 15:50-17:30How neuropixels have accelerated neuroscience data generation: 17:30-19:30…
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1 Precision oncology: an interview with Karina Eterovic 11:56
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In this In the Zone podcast, we explore how precision oncology is currently utilized in the bioanalytical field, the use of liquid biopsies to support these studies and the major challenges impacting drug development.
Hello and welcome to the latest episode of OC Talks, the podcast from Oncology Central. I am Jade Parker, Senior Editor of Oncology Central. Today I am joined by Laetitia Decroix Guilloux, Vice President of Oncology and EMEA Commercial Strategy Lead at Janssen, to discuss the patient journey through lung cancer, examine the lung cancer paradigm, and how the cancer research community can holistically address unmet needs in cancer.…
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1 Tech Blast | CAR-T cell therapy development: from personalized to off the shelf approaches 18:11
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In this Tech blast episode, we talk CAR-T cell development for cancer immunotherapies with Ritwika Biswas, Product Scientist for Sino Biological. We cover some of the latest developments in the field, the advantages of CAR-T over other immunotherapies and selecting the perfect CAR-T antigen, amongst a whole host of different aspects in the field. Contents Introduction: 00:00-00:30 Core principles of cancer immunotherapy: 00:30-02:00 Explaining cancer immunity: 02:00-03:20 Distinguishing CAR-T from native T cells: 03:20-04:40 CAR-T antigen targets: 04:40-06:00 Techniques for engineering CAR-T cells: 06:00-08:00 Introducing off the shelf CAR-T cells: 08:00-09:45 Addressing challenges in off the shelf CAR-T: 09:45-11:30 Current status of CAR-T therapies: 11:30-12:55 Advantages of CAR-T cell therapy over other immunotherapies: 12:55-15:25 The future of CAR-T: 15:10-18:10…
NNM-2021-0076 podcast by Taylor & Francis Group
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1 OCTalks: monitoring MRD in hematological malignancy management 16:03
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In this interview we explore what minimal residual disease (MRD) is in hematological malignancies and how MRD is currently monitored. The interviewees also explain their recent research published in Nanomedicine, which investigates a novel tool to monitor hematological malignancies.
This month on Editor’s Picks, hear about the impacts of aerobic exercise on tumor microenvironments, the science behind female pleasure, how bacteria could be used to make whipped cream and the bacteria that could survive 280 million years on Mars!
In this Tech Blast episode supported by Sartorius (Göttingen, Germany), we talk to Daryl Cole about cell-line development (CLD), some of its challenges and a possible solution: the Octet Bio-Layer Interferometry (BLI) system. Daryl Cole is a Scientist in the Bio Applications Team at Sartorius with extensive experience in a range of fields; he offers insights into the possible applications of CLD and how to get the most out of the Octet BLI system.…
In this episode of Editor’s Picks, we bring you the latest news in the life sciences including DishBrain, a dish of neurons that can play a computer game of table tennis, and click chemistry, which was awarded the Nobel Prize in Chemistry this year.
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1 Incorporating incubation monitoring into your cell culture and analysis workflow 11:26
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In this podcast, we spoke with Katja Piltti from University of California, Irvine (USA) to learn about the importance of quality control in regenerative medicine. We discussed how automated cell culture monitoring and quality control can address changing needs in the transition from preclinical research to cGMP production.…
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1 Tech Blast | Organoids in drug discovery 16:45
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Organoids are transforming the way we approach the drug discovery pipeline. In this Tech Blast episode, Ritwika Biswas, Product Scientist at Sino Biological (Beijing, China), explains why drug discovery needs new methods and how organoids can help. Find out the importance of growth factors in the development of organoids for drug discovery, the challenges associated with their use and the impact of organoid biobanks on the field in this 15-minute overview of the landscape of organoids in drug discovery. Contents: Introductions: 00:00-00:28 Reviewing the drug discovery process: 00:28-02:45 Traditional methods used for target validation and drug screening: 02:45-05:20 Challenges associated with traditional methods: 05:20-07:00 How organoids can be used to address these challenges: 07:00-09:30 The importance of growth factors in organoid development: 09:30-10:50 Challenges in the utilization of organoids in drug discovery: 10:50-13:30 Organoid biobanks: 13:30-14:40 The impact of organoids in precision medicine: 14:40-16:20…
In this Tech Blast episode, we discuss multiplex PCR methods, their challenges and advantages, and the tools being developed to advance multiplexing capabilities. Marco Bianchi, Product Manager at Bio-Rad Laboratories (CA, USA), provides an insight into what role multiplexing plays in research.
In this month's episode of Editor's Picks, we’ve got articles on magnetic synthetic cells, the synthesis of the most complete artificial microbiome yet and how a key protein in human fertilization was identified.
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1 Microbiome profiling techniques: from immunology to agriculture 22:10
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In this episode of the Talking Techniques podcast, we explore microbiomes and the techniques used to profile them. Get an overview of the different techniques’ working principles, their pros and cons and the different applications that they are best suited to. Providing an expert insight into this field is Annabelle Damerum, Microbiome R&D Scientist at Zymo Research. Annabelle reveals some key tips best practice when profiling the microbiome and details the importance of an emerging aspect of the field: the vaginal microbiome. Contents: Into: 00:00-01:50Why it is important to profile microbiomes. 01:50-04:15Key applications of microbiome profiling: 04:15-06:10Techniques used to profile the microbiome and their working principles: 06:10-09:20Targeted sequencing: 06:10-08:00Shotgun metagenomics: 08:00-09:00Metatranscriptomics: 09:00-9:15The strengths and limitations of these techniques: 09:12- Targeted sequencing: 09:20-10.15Shotgun metagenomics: 10:15-11:25Metatranscriptomics: 11:25-11:50Matching techniques with applications: 11:50-13:30Tips for best practice for using these techniques: 13:30-16:25What is one thing you would ask for to improve the ability of microbiome profiling techniques: 16:55-18:10Investigating the vaginal microbiome: 18:10-20:55Conclusions and closing: 20:55-22:10…
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1 Ensuring reliable reagents and robust relationships in cell therapy 30:14
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As the cell and gene therapy industry expands further into commercialization, researchers, developers and manufacturers are growing increasingly aware of the need for specialized ancillary materials in the production of therapeutics. High-quality ancillary materials are often neglected during the early stages of development due to perceived cost, but conversion at later stages of development can be prohibitive and detrimental to program success. In this podcast, in association with Sexton Biotechnologies (now part of BioLife Solutions; WA, USA), Steve Elimann of Orbsen Therapeutics (Galway, Ireland) and Steven Thompson of Sexton Biotechnologies discuss the challenges of variability in producing cell therapeutics, and how using consistent reagents from reliable manufacturers can tackle this challenge. We also dive deep into the importance of gaining and maintaining trustworthy partners early in your cell therapy journey and how this can influence the overall success of your final product.…
In this special episode of Talking Techniques, brought to you from the Federation of European Neuroscience Societies Forum (FENS; 9th–13th July 2022), an expert panel discuss their research into the relationship between the gut–brain axis and addiction. The panel features Benjamin Boutrel (Lausanne University Hospital; Switzerland), Lorenzo Leggio (NIH Intramural Research Program; MD, USA) and Nathalie Delzenne (University of Louvain; Louvain-la-Neuve, Belgium), who discuss their current research into the role of the microbiota in alcohol addiction, if this work could be translated into the clinic, and if similar processes are at play in other addictions, such as food and cocaine. The discussion also features an audience Q&A, which explores the microbial relationship between mother and fetus, and social factors in addiction. Contents:Introduction: 00:00–01:35Introduction to panelists: 01:35–03:40Techniques being used to investigate the relationship between the gut and addiction: 03:40–06:55Translating this research to the clinic: 06:55–11:40Parallels between alcohol addiction and other addictions, such as food, cocaine and tobacco: 11:40–17:05Microorganisms as drivers of behavior: 17:05–18:30The hot topic of gut health: 18:30–20:30Future directions for the research, including microbiota transfers, precision medicine and avoiding alcohol dependence: 20:30–24:35Audience Q&A – microbial elements between mother and fetus, and social factors in addiction: 24:35–29:15…
In this Tech Blast episode, we discuss all things organoids with Ritwika Biswas, Product Specialist at Sino Biological US Inc (PA, USA). Ritwika provides an overview of what are organoids are, how reconbinant growths factors are utlized in their development, the benefits 3D organoid systems offer over 2D cell culture systems and the various applications organoids can be used for. Ritwika also explores the challenges that remain in organoid research and how these can be overcome, as well as what the future holds for these models. Contents: Introduction: 00:00–00:30 What are organoids? 00:30–01:05 Existing organoids: 01:05–02:00 Generating organoids and cell culture methods: 02:00–04:30 Applications of organoids: 04:30–06:10 Comparing 3D and 2D culture systems: 06:10–07:40 Reproducibility, matrigels and increasing complexity: 07:40–09:50 The future of organoids: 09:50–12:20…
Hello and welcome to today’s latest episode of OC Talks Podcast by Oncology Central. My name is Jade Parker, Senior Editor of Oncology Central, and I am joined by Volkmar Mϋller from University Medical Center Hamburg-Eppendorf in Germany. Today we are going to discuss HER2-positive breast cancer including the unmet needs of the disease, and our hopes for the future.…
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1 Tech Blast | Single domain antibodies 16:20
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In this Tech Blast episode, BioTechniques' Digital Editor, Annie Coulson, discusses single domain antibodies with ProSci’s Andy Lee, Lead Scientist of the Single Domain Antibody Program, and David Eling, Director of Business Development. Andy and David describe what single domain antibodies (sdAbs) are, including their unique characteristics, such as their small size. They then go on to explore the applications of sdAbs, from increasing resolution in imaging studies, to developing antidotes for snake venom. Listen to this episode to get an overview of sdAbs, how they could be incorporated into your research and their link to llamas. Contents: Introductions: 00:00–01:25 What are single domain antibodies? 01:25–3:05 Applications of single domain antibodies: 03:05–05:05 Unique characteristics of sdAbs: 05:05–07:55 sdAbs for SARS-CoV-2: 07:55–08:35 sdAbs for therapeutics and diagnostics: 08:35–11:25 Unique applications, including biosensors and animal feed: 11:25–12:50 Working with researchers to develop sdAbs: 12:50–13:25 The future of sdAbs: 13:25–15:50 Closing remarks: 15:50–16:20…
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1 Tech Blast | Comprehensive genomic profiling in cancer 16:10
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In this Tech Blast episode, our Digital Editor Annie Coulson talks with Carlo Messina, Senior Director for Oncology in Global Development and Medical Affairs at Roche (Basel, Switzerland), about the use of comprehensive genomic profiling (CGP) in cancer research. CGP can obtain more information about a tumor from a smaller biospecimen compared with other genomic profiling techniques and gives oncologists more tools for treating patients. Carlo talks about the applications of CGP, including detecting genomic aberrations in the bloodstream, and the future of this technique.…
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1 3D cell cultures: the latest developments, applications and regulations in the field 30:22
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In this episode, supported by BrandTech, returning guest Rob Vries, CEO of HUB organoids (Utrecht, The Netherlands), fills us in on the advancement of 3D cell cultures and organoids over the last 2 years, starting off by documenting how the pandemic impacted their development and uptake. We also discuss how the recent FDA Modernization Act, removing the requirement for drug candidates to be tested on animals, has impacted the drive for improved models, the key techniques available to analyze them and what still needs to change for 3D cell cultures to fully replace animal models in the lab. Listen today to find out which resources can help you begin to work with 3D cell cultures, their most exciting recent applications and how issues of reproducibility are currently being addressed in the field. Contents:Intro: 00:00-00:50How COVID-19 impacted the uptake and development of 3D cell cultures: 00:50-02:05Key developments in 3D cell culture technology in the last 2 years: 02:05-03:00Intestinal organoids and the investigation of irritable bowel syndrome: 03:00-5:50Evaluating assembloids: 05:50-08:35Complexity vs clinical relevance: 08:35-10:45The impact of 3D cell cultures in precision medicine in cancer: 10:45-12:40The impact of the FDA’s Modernization Act on 3D cell culture uptake: 12:40-14:45What needs to improve in organoid technologies to fully replace animal models? 14:45-16:10Changing inbuilt reliance on animal models: 16:10-18:30Key techniques to analyze organoids: 18:30-19:50Developments in imaging technology that have improved the analysis of organoids: 19:50-21:15Current challenges in 3D cell culture implementation and reproducibility: 21:15-24:15Improving access to 3D cell cultures: 24:15-25:55Resources to help people implement organoids into their work: 25:55-27:15Searching for the holy grail in 3D cell cultures: 27:15-28:00…
In this episode of Editor’s Picks, presented by guest-host Grace Tyrrell, learn about how the need for vaccine refrigeration could be on the way out, a new chemical approach that may aid in the fight against antibiotic resistance and the use of AI algorithms as a potential cure for drug-resistant epilepsy.…
Welcome to OC talks, a podcast series exploring the latest advancements in oncology. I’m your host, Jade Parker, Oncology Central’s Senior Editor. In this episode, supported by Sengenics, we will be exploring the importance of autoantibodies in the development of cancer as well as how they can be used in the early detection of tumors. Joining me for this episode is Field Application Scientist Joseph Miseirvitch from Sengenics, a precision medicine company working to improve patient outcomes for physiologically relevant, data-guided decision making.…
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1 BZ PPD Spotlight Podcast: an interview with Cyrille Bonhomme 19:25
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BZ PPD Spotlight Podcast: an interview with Cyrille Bonhomme by Taylor & Francis Group
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1 rAAVs, host cell contamination and ddPCR 26:39
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In this episode of Talking Techniques, supported by Bio-Rad, we discuss a key component of many gene therapies: recombinant adeno-associated viruses (rAAVs) and their production. These viruses act as efficient, accurate delivery vesicles for the gene therapy’s plasmid. Speaking to Associate Director of Biopharma Product Marketing at Bio-Rad Laboratories, Mark White, we take a look the different expression systems used for their production and compare their advantages, before looking at some of the challenges involved in the production rAAVs, such as host cell contamination. Discover the tools that can help minimize host cell contamination and differentiate between nuclease resistant and nuclease reactive contaminant DNA and find out about some of the most exciting developments in rAAV technologies. Contents:The role of rAAVs in gene therapies: 00:40-02:15 The production of rAAVs and gene therapies: 02:15-03:30 Why are HEK cells so popular for cell therapy production? 03:30-05:45 HEK vs SF9 Insect cell expression systems: 05:45-06:45 Challenges in cell therapy expression systems: 06:45-08:05 Host DNA contamination: 08:05-10:30 The risks of host DNA contamination: 10:30-12:45 Key techniques to minimize host DNA contamination: 12:45-14:40 The advantages of ddPCR in gene therapy production: 14:40-17:50 Distinguishing between nuclease resistant and nuclease reactive host cell DNA: 17:50-19:10 The most exciting developments in rAAV technology: 19:10-20:20 What is one thing you would ask for to improve rAAV and gene therapy development 20:20-26:38…
In this episode of Editor’s Picks, hosted by our Assistant Editor Aisha Al-Janabi, learn about recent concerns over the validity of a pivotal Alzheimer’s disease study, how researchers answered a question about the heart dating back to the 1600s and a laser-based diamond sensing technology that improves magnetoencephalography precision.…
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1 HPV, epigenetics and cancer: sequencing for new insights and to correct healthcare inequality 17:00
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In many regions of the world, HPV and its association with cervical cancer is a well-known but rarely considered issue, after the rollout of the HPV vaccine in the late 2000s. However, while this rollout significantly impacted cervical cancer rates where it was implemented, it was not universal and in many areas of the world HPV induced cervical cancer remains a critical issue. Cervical cancer is the leading cause of death for people with a cervix living in poverty in low-resource regions and on the continent of Africa, it is estimated that in 2020 as many people with a cervix died of cervical cancer as COVID-19. In this episode of Talking techniques, supported by Oxford Nanopore, Senior Investigator Michael Dean and Post-baccalaureate Fellow Nicole Rossi, from the NIH’s National Cancer Institute (MD, USA), discuss their research into the relationship between HPV and cervical cancer. Expounding on the points mentioned above, they reveal the tremendous insights into cancer and immunology still to be gained, document the key tools used in their studies and explain how their work can be translated into more effective immunotherapies and treatments for cervical cancer. ContentsIntroduction: 00:00-01:40How big an issue does HPV present to the world? 01:40-03:30HPV’s association with cancer: 03:30-05:15Why does HPV target DNA repair mechanisms? 05:15-06:00Key goals of research into HPV: 06:00-07:15Challenges of HPV research: 07:15- 08:20Solutions available to deal with these challenges: 08:20-09:30The most exciting discoveries in HPV using long-read sequencing: 09:30-11:00HPV16- the most oncogenic variant: 11:00-11:40Translating research into clinical results for HPV: 11:40-12:45How effective is the current HPV vaccine? 12:45-13:30What would you ask for to improve research into HPV and cancer? 13:30-14:30How big an issue is vaccine rejection? 14:30-15:40Final thoughts: 15:40-17:00…
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1 Artificial intelligence in healthcare 30:29
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Recently, the Alliance for Artificial Intelligence in Healthcare (AAIH) published "The Lifecycle of an AI System in Healthcare", a whitepaper defining what healthcare is and provides guidelines on implementing artificial intelligence (AI) and machine learning (ML) in this setting. In this episode of Talking Techniques, we speak with Oscar Rodriguez, a board member at AAIH and one of the authors of this whitepaper, to find out more about what the lifecycle of AI is, the importance of having guidelines when applying AI or ML in healthcare and what the future holds for this type of computer modeling. Contents: Intro: 00:00 - 00:45What are some of the ways that AI is used in healthcare? 00:45 - 5:04What are the current limitations of using AI in healthcare settings? 5:04 - 9:28The lifecycle of an AI system: 9:28 - 14:04How did you develop these guidelines for AI in healthcare? 14:04 - 17:28Summary of the guidelines from the white paper: 17:28 - 19:09Do you think some of these guidelines could be applicable to AI outside of healthcare? 19:09 - 19:53Why is it important to have guidelines like these for AI and machine learning in healthcare? 19:53 - 22:11Why did you decide to focus on COVID-19 case studies? 22:11 - 23:05How do you think the pandemic has changed the way AI is used in healthcare? 23:05 - 26:26What's next for AI? 26:26 - 30:03…
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1 Artificial intelligence in healthcare 30:29
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Recently, the Alliance for Artificial Intelligence in Healthcare (AAIH) published "The Lifecycle of an AI System in Healthcare", a whitepaper defining what healthcare is and provides guidelines on implementing artificial intelligence (AI) and machine learning (ML) in this setting. In this episode of Talking Techniques, we speak with Oscar Rodriguez, a board member at AAIH and one of the authors of this whitepaper, to find out more about what the lifecycle of AI is, the importance of having guidelines when applying AI or ML in healthcare and what the future holds for this type of computer modeling. Contents: Intro: 00:00 - 00:45What are some of the ways that AI is used in healthcare? 00:45 - 5:04What are the current limitations of using AI in healthcare settings? 5:04 - 9:28The lifecycle of an AI system: 9:28 - 14:04How did you develop these guidelines for AI in healthcare? 14:04 - 17:28Summary of the guidelines from the white paper: 17:28 - 19:09Do you think some of these guidelines could be applicable to AI outside of healthcare? 19:09 - 19:53Why is it important to have guidelines like these for AI and machine learning in healthcare? 19:53 - 22:11Why did you decide to focus on COVID-19 case studies? 22:11 - 23:05How do you think the pandemic has changed the way AI is used in healthcare? 23:05 - 26:26What's next for AI? 26:26 - 30:03…
Welcome to our newest podcast series, Editor's Picks, where we'll round up the latest life science news from BioTechniques. In this bitesize podcast, we've got everything from the history of cephalopods in neuroscience research to recent advancements against antibiotic-resistant bacteria.
Mitigation strategies for the biological matrix supply chain: an interview with Mark O'Dell by Taylor & Francis Group
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1 Tech Blast | Sculpting bioinformatics workflows for NGS data analysis 15:50
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In this episode, supported by Zymo Research, guest host Georgia Bickerton explores NGS data and how bioinformatic pipelines and workflows can be optimized to make NGS data more accessible. To do this she speaks with Jeffrey Bhasin, Director of Informatics at Zymo Research, who discusses the key issues of reproducibility in bioinformatics, the selection of methods available to analyze different datasets and to investigate different aspects of these data. Listen today to discover the techniques available to help increase the portability and reproducibility of fine-tuned bioinformatics workflows and to streamline the application of these workflows. Bhasin also reveals how these 'workflow management' technologies can enable more global, collaborative approaches to bioinformatics workflow construction and begin to increase the automation and accessibility of bioinformatics workflows. Contents: Introduction: 00:00-00:55 How the rise of NGS has impacted the field of bioinformatics: 00:55-01:35 Issues of reproducibility in bioinformatics: 01:35-03:50 Using bioinformatics for different data types and different applications: 03:50-06:10 The impact of bioinformatics workflow management: 06:10-08:50 The future of NGS data analysis: 08:50-12:05 Busting overreliance on bioinformatics experts? 12:05-15:05 Closing remarks: 15:05-15:50…
ADA assay development: a podcast interview with Lynn Kamen by Taylor & Francis Group
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1 Liquid biopsy and cfNAs: driving forward diagnostics and disease research 22:16
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The key diagnostic and prognostic information locked away in cell-free nucleic acids (cfNAs) has become increasingly accessible due to developments in genetic and epigenetic profiling techniques. These advances have engendered the rise of liquid biopsy techniques, which capture and analyze cfNAs from samples such as blood, saliva, urine and feces, in diagnostics and basic disease research. However, challenges remain in the detection and analysis of these nucleic acid fragments, in part due to their low abundance and fragile nature. In this episode, Ayla Maunighan-Peter, Epigenetics Product Specialist at Zymo research (CA, USA), details the utility of these molecules, the challenges associated with their development and implementation of liquid biopsies and their use in both basic research and diagnostic spaces. For an insight into how cfNAs can be used to identify novel drug targets, impact diagnostics development and be used to help soothe the organ shortage crisis, listen today! Contents: Intro: 00:00-00:40What are cfNAs? 1:50-02:35The role of cfNAs in Liquid biopsy: 02:35-04:25Markers analyzed in cfNAs: 04:25-06:10Epigenetic analysis of cfNAs: 06:05-07:55Techniques for epigenetic analysis of cfNAs: 08:00-08:55Introducing fragmentomics: 08:55-11:05Challenges in the development of liquid biopsy: 11:05-14:40How is the field trying to adapt to these challenges? 14:40-16:10Applications of cfNAs in basic research: 16:10-17:30The most exciting findings concerning the role of cfNAs in disease: 17:30-20:05One thing to improve our understanding and use of cfNAs: 20:05-21:10Conclusions: 21:15-22:15…
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1 Liquid biopsy and cfNAs: driving forward diagnostics and disease research 22:12
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The key diagnostic and prognostic information locked away in cell-free nucleic acids (cfNAs) has become increasingly accessible due to developments in genetic and epigenetic profiling techniques. These advances have engendered the rise of liquid biopsy techniques, which capture and analyze cfNAs from samples such as blood, saliva, urine and feces, in diagnostics and basic disease research. However, challenges remain in the detection and analysis of these nucleic acid fragments, in part due to their low abundance and fragile nature. In this episode, Ayla Maunighan-Peter, Epigenetics Product Specialist at Zymo research (CA, USA), details the utility of these molecules, the challenges associated with their development and implementation of liquid biopsies and their use in both basic research and diagnostic spaces. For an insight into how cfNAs can be used to identify novel drug targets, impact diagnostics development and be used to help soothe the organ shortage crisis, listen today! Contents: Intro: 00:00-00:40What are cfNAs? 1:50-02:35The role of cfNAs in Liquid biopsy: 02:35-04:25Markers analyzed in cfNAs: 04:25-06:10Epigenetic analysis of cfNAs: 06:05-07:55Techniques for epigenetic analysis of cfNAs: 08:00-08:55Introducing fragmentomics: 08:55-11:05 Challenges in the development of liquid biopsy: 11:05-14:40How is the field trying to adapt to these challenges? 14:40-16:10Applications of cfNAs in basic research: 16:10-17:30The most exciting findings concerning the role of cfNAs in disease: 17:30-20:05One thing to improve our understanding and use of cfNAs: 20:05-21:10Conclusions: 21:15-22:15…
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1 Tech Blast | Organ-on-a-chip models of fatty liver disease 14:33
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In this Tech Blast episode, we discuss preclinical models of non-alcoholic fatty liver disease with Gareth Guenigault, Lead Scientist of Services at CN Bio (Cambridge, UK). Gareth provides an overview of non-alcoholic steatohepatitis (NASH) therapeutic landscape and explains how incorporating organ-on-a-chip models into drug discovery workflows can improve this landscape. Get Gareth’s expert insight into the field and discover how to develop liver-on-a-chip NASH models, tips for analyzing the resultant data, and the future of organ-on-a-chip technologies. Contents: Introduction: 00:00–00:30 Pathology and prevalence of NASH: 00:30–01:50 Challenges faced when developing drugs for NASH: 01:50–03:40 An overview of organ-on-a-chip models: 03:40–05:05 Liver-on-a-chip models for NASH: 05:05–07:05 Incorporating organ-on-a-chip into drug discovery workflows: 07:05–08:45 Accessing liver-on-a-chip and NASH models: 08:45–09:45 The NASH-in-a-box workflow: 09:45–10:45 Gareth’s tips for analyzing the data: 10:45–12:15 The future of organ-on-a-chip technology: 12:15–13:55…
FON-2021-1277 OCTalks by Taylor & Francis Group
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1 The evolution of recombinant antibodies 17:58
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In this episode, we discuss recombinant proteins: what are they and what are they used for? BioTechniques Editor Tristan Free speaks to Amy Sheng, Technical Account Manager at Sino Biological, who explains the history of recombinant proteins and how protein modeling techniques such as AlphaFold are changing their development. Find out how monoclonal antibodies have been used through the COVID-19 pandemic and how we may overcome challenges in production, upscaling and more in this episode of Talking Techniques. Contents:Introduction 00:00-01:26What are recombinant proteins and what are they used for? 01:26 – 02:42How were they first developed? 02:42 – 03:50Since initial development has recombinant antibody development progressed today? 03:50 – 05:57What are the advantages of utilizing the more traditional mouse monoclonal antibodies? 05:57 – 07:11Are recombinant antibodies used in more specialist applications and are they more likely to be used in therapeutics? 07:11 – 09:47How have AI systems like AlphaFold impacted the development of recombinant antibodies? 09:47 – 12:07How has antibody production changed and what’s next? 12:07 – End…
In this Tech Blast episode, we discuss generating, standardizing and sharing microbiome data with Raul Cano, Chief Scientific Officer at The BioCollective. Raul provides a brief overview of the reproducibility crisis and explores how the use of standards and databases can address the challenges it poses. Get Raul’s insight into the field and discover the steps that can be taken to make microbiome research accessible and reproducible. Contents: Introduction: 00:00–00:40 Overview of the reproducibility crisis: 00:40–02:25 The importance of standards for microbiome data: 02:25–05:05 Sharing microbiome data: 05:05–07:10 Comparing microbiome databases: 07:10–08:20 The significance of metadata: 08:20–08:50 How databases can enhance the use of standards: 08:50–10:05 Databases help standardize microbiome research: 10:05–10:45 Benefits of sharing data: 10:45–11:40…
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1 3D and tea: Dolores Serrano and Katerina Lalatsa on 3D printing in cancer studies 24:09
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In this '3D and tea' interview, we speak to Dolores Serrano and Katerina Lalatsa all about the potential of utilizing 3D printing in cancer studies.
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1 NCTalks with Jan Hillert: pregnancy outcomes in MS 14:17
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NCTalks with Jan Hillert: pregnancy outcomes in MS by Taylor & Francis Group
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1 Flow cytometry for CGT assay development: an interview with Richard Cuthbert 11:21
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Flow cytometry for CGT assay development: an interview with Richard Cuthbert by Taylor & Francis Group
In this podcast, Andrew Hall (Labcorp, UK) discusses the evolution of large molecule bioanalysis workflow automation as well as the present challenges facing the industry.
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1 Bioanalytical assays for gene therapy programs: an interview with Catherine Vrentas 14:09
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Bioanalytical assays for gene therapy programs: an interview with Catherine Vrentas by Taylor & Francis Group
In this episode, supported by Zymo Research, we discuss the epigenetic clock: what is it? How are researchers using it and what are some of the key tools available to study it? Elucidating the link between epigenetics and aging is Keith Booher, Director of Services at Zymo research including aging and epigenetics research services. Keith reveals how our behaviors and environment, such as diet, exercise and air quality, can impact our epigenetics and the impact that the epigenetic clock has played in the development of new therapeutics. Find out how you can reverse your biological age, the implications of aging populations the latest developments in epigenetic research and more in this episode of Talking Techniques. Contents: Introduction: 00:00-02:15The issue with population aging: 02:15-03:30Introducing epigenetics: 03:30-04:20The link between epigenetics and aging: 04:20-05:50The impact of exercise on epigenetics: 05:50-06:40The epigenetic clock: 06:40-08:00What can the epigenetic clock tell you about someone’s health: 08:00-10:05Can you reverse the epigenetic clock? 10:05-12:00The latest experiments in epigenetics and aging research: 12:00-14:10Finetuning exercise using the epigenetic clock: 14:10-15:30Challenges in epigenetics and aging research: 15:30-17:00Key technologies in epigenetics and aging research: 17:00-18:05Where are the main developments coming from? 18:05-19:30Aging and lobsters: 19:30-20:45How will the field develop in the next 5 years? 20:45-22:00What would you ask for to improve your understanding of the epigenetic clock? 22:00-23:00The most exciting aspects of aging intervention: 23:00-24:00…
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1 ddPCR for CGT quality control: a podcast with Mark White 15:00
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ddPCR for CGT quality control: a podcast with Mark White by Taylor & Francis Group
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1 Hybrid assays: an interview with Barry Jones and Adriane Spytko 11:14
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In this podcast Barry Jones (Director, LC–MS Biologics and Biomarkers at Q² Solutions) and Adriane Spytko (Manager, LC–MS Biologics at Q² Solutions; both NY, USA) discuss hybrid assays using both LBA and LC−MS techniques. They share their thoughts on bottom-up LC−MS methods and the concern about detecting analyte that is not intact as well as future projects that bridge LC−MS and LBA.…
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1 Resolving spatial biology in neuroscience 30:23
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In this episode of Talking Techniques, supported by Resolve BioSciences, we explore the realm of spatial biology, with a particular focus on the techniques involved in this approach to biological exploration, and the exciting insights gleaned from these techniques that have led to this approach being labeled Natures Method of the year 2020! Stefano Pupe, Postdoctoral Researcher at German Center for Neurodegenerative Diseases, takes me through this field, revealing – amongst other fascinating insights – the neuroscience studies that can now be conducted on live mice to monitor their neurological cell expression profiles as they perform specific behaviors. We also discuss the potential immortality complexes that could be driving a large amount of investment into the field and highlight some of the challenges to watch out for when conducting spatial biology studies. ContentsIntroduction 00:00-02:20 The concept of spatial biology 02:20-03:30 The key techniques involved in spatial biology: 03:30-05:10 What does the combination of transcriptomics and proteomic profiling enable you to determine: 05.10-06:00 Unique insights from spatial biology: 06:10-07:20 Spatial biology in the characterization of cells from the medial septal: 07:20-8:20 First impressions of working with spatial biology: 8:10-09:20 How many omic datasets you can generate from one tissue sample? 09:20-10:45 The impact of spatial biology in diagnostics: 10:45-11:50 Exciting breakthroughs in neuroscience from spatial biology: 11:50-14:00 Predicting neuronal activity with spatial data: 14:00-15:55 Developments in microscopy pairing with spatial biology: 15:55-18:05 The challenges of working with a new technique: 18:05-19:45 The role of automation in resolving with vast data sets 19:45-21:45 Why Facebook and Google are looking assisting with spatial biology: 21:45-24:15 What would you ask for to take spatial transcriptomics to the next level? 24:15-25:40 Challenges of reproducibility in an emerging field: 25:40-29:05 Final comments: 29:05-30:25…
In this episode of Talking Techniques, we introduce a new podcast series, produced by BioTechnques and hosted by the enigmatic Antentor Othrell Hinton Jr, Assistant Professor of Molecular Physiology and Biophysics at the Vanderbilt University, and bring you a sneak peek of the first episode of the series. In this podcast series, Antentor will discuss all things diversity, equity and inclusion in STEM, meeting with different guests each episode from across the spectrum of fields and people in the research community. Each episode will keep science at its core, exploring each guest's research and finding out how they encourage open and productive environments in their working communities. In episode one, dive into the discussion around mentoring practices and with our first guest, Christina Termini Assistant Professor a the Fred Hutch Cancer Research center (CA, USA) Contents: Introduction: 00:00-02:40 Christina's Research in hematopoietic stem cells and cancer treatments. 02:40-05:45 Molecular regulators of the hematopoietic system: 05:45-06:20 Building mentoring networks and addressing microaggressions: 06:20-09:00 Isolation in academic spaces and countermeasures: 09:00-10:35 The community of scholars and the 1000 Black scientists lists: 10:35-17:00 What are you drinking? 17:00-17:40 Mental health in mentoring: 17:40- 20:45 The work of the American Society of Cell Biology developing mentors: 20:45-23:00 Equalling opportunities with virtual and non-traditional faculty interviews: 23:00-26:50 Building the 100 Latinx list: 26:50-29:30 Contact Tina! 29:30-31:55 Coming up on STEM Tea! 32:10-34:54…
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1 Cell proliferation in drug screening 20:41
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In this episode, supported by BioRad, we explore cell proliferation monitoring and its role in drug discovery, guided by the expert advice of Rachel Preston, Product Development researcher at Bio-Rad. We discuss techniques available to monitor cell proliferation, their advantages and disadvantages and the importance of selecting the right combination of techniques for different experimental targets. Rachel also provides some tips for best practice and some pitfalls to avoid regarding DNA damage and highlights the importance of good quality antibodies in cell proliferation assays. Don’t miss out on the most exciting applications of cell proliferation today, and find out what still needs to be improved for cell proliferation assays to continue driving drug discovery forward; all in the first episode of Season 3 of Talking Techniques! Contents: Intro: 00:00-00:34Why measure cell proliferation in drug discovery? 00:34-2:20Techniques involved in the detection of cell proliferation: 02:20-04:20The advantages and disadvantages of different techniques: 04:20-06:50 Selecting the most appropriate technique: 06:50-09:30How do you account for DNA damage in these assays? 09:30-11:00The best combination of techniques for drug screening studies: 11:00-12:00The biggest developments in technologies for cell proliferation monitoring: 12:00-14:15 Exciting findings of cell proliferation studies in cancer research: 14:15-15:35What element of cell proliferation detection and monitoring would you choose to improve? 15:35-17:40Closing remarks: 17:40-19:10…
In this Tech Blast episode, is Jay Lemke, Senior Research Scientist at Promega, provides a brief overview of Sanger sequencing, its history, working principle and development over the last half century. Jay reveals which lab practices are still are best completed with Sanger sequencing and explains why the technique remains so prevalent today, even in the era of next-generation sequencing. Get Jay's top tips for best practices when working with sanger and much more throughout the episode! Contents: Introduction: 00:00-00:40 A brief history of Sanger sequencing: 00:40-01:00 Sanger sequencing working principle: 01:00-03:00 How has sanger developed since it was invented? 03:00-05:10 The advantages of Sanger in an era of NGS: 05:10-07:00 Best applications of Sanger sequencing: 07:00-08:45 Top tips for success with sanger: 09:30-10:10 Challenges of working with sanger and addressing them: 10:10-11:35 What would you improve about sanger sequencing? 11:35-12:25…
Find out about current treatment options for thyroid cancer such as the recent approval of selpercatinib for patients with an alteration in the RET gene in this interview with Kate Newbold who is a clinical oncologist consultant at The Royal Marsden Hospital in London (UK).
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1 Synthetic biology: from cancer to the climate crisis 26:01
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In this episode, we explore the field of synthetic biology. Discover the history of the field and its origins in the production of biofuels, before fracking burst onto the scene forcing a pivot in direction towards chemical production. Find out about the key techniques that have brought synthetic biology into the realm of immunotherapies and cancer research, which have even welcomed in a return to applications focussed on tackling the climate crisis, such as lab-grown meat. To investigate this space, I speak with Merrit Savener, Biopharma Technical Account Manager at Molecular Devices (CA, USA) and Adam Clore Technical Director of Synthetic Biology at Integrated DNA Technologies (IA, USA). Merrit and Adam provide their insight into the most exciting applications of synthetic biology, the regulation surrounding it, how they would wish to improve the field, revealing the true impacts that this field can deliver in addressing some of humanity's biggest issues. Contents:Introduction: 00:00-02:30Explaining synthetic biology: 02:30-03:45Origins of synthetic biology: 03:45-05:25Key current applications of synthetic biology: 05:25-07:30 Key methods involved in synthetic biology: 07:20-09:30Challenges in synthetic biology for product production: 09:30-12:35Regulating synthetic biology: 12:35-14:55What would you change to improve synthetic biology? 14355-16:55Most exciting applications of molecular biology: 16:55-20:40 The future of synthetic biology: 20:40-22:45Making electronics manufacturing more sustainable with synthetic biology: 22:45-23:15Changing perceptions in synthetic biology: 23:15-25:30…
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1 Tech Blast | Quality assurance in drug discovery 14:18
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In this Tech Blast episode, Tim Bolus, Compliance Program Manager at Molecular Devices, provides a 15-minute in-depth exploration of the regulatory landscape of drug discovery and development. Tim examines the good lab and manufacturing processes essential to successful drug discovery, development and production. Get Tim's insight into the field and discover how to best prepare your lab to pass the FDA's regulations and hear some firsthand cautionary tales! Contents: Introducing GXP: 00:40-2:20 Why is GLP important in drug development? 2:20-05:55 Why is GMP important in the manufacturing processes late down the line? 05:55-07:15 Examples of GXP going wrong and the ramifications: 07:15-10:30 Solutions to ensure GXP: 10:30-11:40 Aspects of GLP that need improving: 11:40-13:40…
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1 Establishing an epigenetic lens through which to explore infectious disease and cancer 23:40
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We are talking all things DNA methylation in this episode, providing a brief explanation of epigenetics and the technologies involved in the study of DNA methylation, before exploring the application of these techniques in the study of infectious diseases and cancer. Revealing all of this information is my guest Chloé Goldsmith, Postdoctoral Research Fellow at Canberra University, whose research focuses on DNA methylation, Hepatitis B Virus and the link between lifestyle and the epigenome. Chloé explains the strengths and weaknesses of the different techniques used to explore DNA methylation and highlights the advances in long-read sequencing that are dramatically improving this aspect of epigenomic research. Chloé also discusses some of the most interesting insights that she has gained by taking a single-cell and single-molecule approach to her research and highlights some of the recent findings in the field that have caught her attention and broadened our understanding of epigenetics in disease. Listen today to find out what functions remain to be improved for more insightful epigenetic studies and for some key insights into the future of the field. Contents:Introduction: 00:00-01:45 Introducing the epigenetics and the different types of epigenetic regulation: 00:45-02:55 Techniques involved in the exploration of DNA methylation: 03:55-05:50 Challenges of bisulfite conversion techniques: 05:50-07:15 Recent developments in DNA methylation technologies: 07:15-09:40 Long reads vs short reads for epigenetic studies: 09:40-10:45 Epigenetics in infectious disease research: 10:45-13:15 Lifestyle, the epigenome and HBV: 13:15-16:00 Epigenetics in cancer research: 16:00-18:05 Recent developments in our epigenetic understanding of disease: 18:05-19:40 One area of technology that needs to be improved: 19:40-21:50 Aspirations for the field: 21:50-23:25…
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1 Target enrichment for NGS: Why, how and what's next? 18:00
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In this episode, supported by Roche, we explore a vital aspect of many next-generation sequencing studies: target enrichment. Here, regions of interest are selectively amplified before sequencing takes place. More specifically we examine some of the new technologies improving this key step and their impact on oncology research. My guest for this episode is Brian Godwin, Director of Reagent and Assay Development at Roche sequencing. Brian reveals the myriad of ways that poor target enrichment can impact a study while highlighting its importance at the foundations of many research efforts. Discover the key limitations of some target enrichment techniques and the new technologies that are improving this step, leading to a new suite of applications in oncology research, where damaged or degraded DNA samples are common. Listen today for an overview of the target enrichment process, the most exciting applications of this technique future potential of this step. Contents:Intro: 00:00-01:30Why conduct target enrichment? 00:40-02:45Established technologies for target enrichment: 02:45-04:10Challenges of working with these technologies: 04:20-06:40The impact of these challenges on sequencing results: 06:40-07:35New technologies to address these challenges: 07:35-09:25Primer extension in PCR and target enrichment: 09:25-11:40Exciting applications of primer extension target enrichment: 11:40-13:25Improving target enrichment in oncology and its impact on the field: 13:25-16:15If you could ask for one thing to enable primer extension target enrichment to make the greatest possible impact on the NGS and oncology communities, what would it be? 16:15-18:00…
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1 Producing challenging proteins in the golden age of protein engineering 19:58
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In this episode, we roam the world of recombinant proteins and their production, discussing the challenges of their production, and recent evolutions in protein engineering that have brought the field into a golden era of innovation. I'm joined by recombinant protein engineering expert Yuning Chen, R&D Manager at Sino Biological, who discusses the key applications of recombinant proteins, the aspects of certain products that make them difficult to produce and how these difficulties can be overcome. Yuning also reveals how the manipulation of antibodies has been essential to multiple aspects of our response to the COVID-19 pandemic. After a review of the recent evolutions in protein engineering, from cell-free systems to automation, Yuning describes his vision for the future of the field and reveals his support for the Terminator-style uprising that he believes could revolutionize it. ContentsThe production of recombinant proteins: 00:48-02:11 The key applications of recombinant proteins: 02:11-03:27 What makes some proteins difficult to produce? 03:27-04:41 Overcoming these challenges: 04:40-5:40 Manipulating antibodies to take part in multiple aspects of pandemic response: 05:40-08:00 The most exciting applications of recombinant proteins: 08:00-09:50 Developments in high throughput and automated protein engineering: 09:50-11:00 Cell-free protein engineering: 11:00-12:40 The benefits of cell-free systems: 12:40-13:40 What is next for recombinant protein production? 13:40-16:15 The dream of automation for the recombinant protein production: 16:15-18:50…
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1 Analysis of nitrosamine impurities: an interview with Javier Jimenez-Villarin 12:54
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Analysis of nitrosamine impurities: an interview with Javier Jimenez-Villarin by Taylor & Francis Group
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1 The evolution of PCR: From q to dd and beyond 24:50
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PCR has evolved rapidly over the last few years, an evolution that has been dramatically accelerated by the COVID-19 pandemic. New technologies and approaches have been brought to the fore and the full range of applications for which this technique can be applied have been highlighted, from diagnostics to basic disease research and immunology. This episode explores this evolution, looking at how challenges presented by the pandemic forced the hand of researchers to think outside the box and build on lesser-known PCR technologies. Here, Eddy van Collenburg, Market Development Specialist at Bio-Rad, provides his insight into how ddPCR rose to prevalence during this time and explains how the technology is being used to enable trade, detect new variants and is being used outside of the pandemic. Discover the key role that ddPCR can play in gene therapy research, liquid biopsies, cancer research, single-cell studies and more, in this fascinating overview of the development of PCR and where the technique can go in the future. Contents: Introduction: 00:00-01:30Why was qPCR established as the gold standard for detection? 01:30-03:40Limitations of qPCR during the pandemic 03:40-05:05New varieties of PCR for faster results: 05:05-05:40New varieties for more accurate results: 05:40-06:45ddPCR working principle and advantages: 06:45-09:00Nonclinical ddPCR applications for COVID-19: 09:00-10:45ddPCR in variant detection: 10:45-12:05ddPCR in gene therapy research: 11:05-12:30Liquid biopsy and cancer research: 12:30-15:20Combining ddPCR with NGS in research: 15:20-16:15What are some of the applications of ddPCR that you find most exciting? 16:15-17:10 Applications of ddPCR in single-cell studies 17:10-21:00Improvements in ddPCR: 21:00-21:45 Tips for best practice when using ddPCR: 21:45-22:50What would you ask for to improve ddPCR? 21:50-24:20…
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1 Data Completeness: a podcast with Dan Drozd 17:41
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Data Completeness: a podcast with Dan Drozd by Taylor & Francis Group
Love it or hate it, western blotting forms the bedrock of countless studies across numerous disciplines. Explore its history, development and applications in this episode all about the marmite of life science techniques. Guiding me through the hand wringing, hair follicle destroying history and process of western blotting, and hopefully explaining the beauty and potential of the technique - is Kenneth Oh, Senior Project Manager at Bio-Rad Laboratories. Kenneth reveals some of the latest developments in western blotting. Revealing how researchers are now able to validate the success of each stage of a western blot and work with smaller samples, Kenneth provides key tips for ensuring each of your western blots is a triumph. Contents: Intro: 00:00-01:15 History of western blotting: 01:15-3:50 What makes western blotting so tricky? 03:50-05:30 Stepwise optimization: 05:30-06:50 Stain-free western blotting: 06:50-08:50 The different variants of western blotting: 08:50-10:20 The right blot for the right application: 10:20-11:30 The most exciting developments in western bloting:11:30-13:35 New horizons for western blotting: 13:35-15:25 The impact of multiplex-western blotting: 15:25-16:30 Tips for best practice: 16:30-18:10 The future of western blotting: 18:10-20:30 Yearning for automation: 20:30-21:30…
Immunoassay applications: a podcast with John Chappell by Taylor & Francis Group
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1 Molecular therapeutics: how far have we come and what is on the horizon? 37:53
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Uncover the history of molecular therapeutics, the staggering current applications and developments of these therapeutics and what their future could hold, in this episode of Talking Techniques. To explore the field of molecular therapeutics and provide an insight into their production, is Aaron Clauson, Product Manager at Zymo research. First, we cover the ins and outs of targeting the therapies and how to select the correct type of molecular therapeutic to begin developing a drug for a specific disease, before looking at some of the setbacks and safety errors that have occurred during the development of molecular therapeutics and how researchers have learned from these tragic events. Aaron also highlights the vital role of plasmids in almost all molecular therapeutic development, detailing the importance of keeping plasmid solutions and transfections free of endotoxins, revealing key solutions for avoiding these contaminants. Listen today to get all you need to know about molecular therapeutics, from their inception to their most exciting examples and how we can better communicate their benefits to the public in order to avoid mistrust and confusion - as has occurred during the COVID-19 pandemic. Contents:What counts as a molecular therapeutic? 01:45-02:40Well known molecular therapeutics 02:40-05:00What can molecular therapeutics achieve that synthetic chemicals can’t? 05:00-05:55Targeting molecular therapeutics and picking the right type of therapeutic 05:55-08:25Selecting the appropriate molecular therapeutic 08:35-11:00Setbacks and safety concerns in gene therapies 11:00-15:45Preventing off-target effects in gene therapies 15:45-17:35Challenges in the development of molecular therapeutics 17:35-19:05 Plasmids in molecular therapeutic development 19:05-23:55 Avoiding endotoxins: 23:55-27:45What fields do you find most exciting? 27:45-28:50Better communication of gene therapies to the public 28:50-32:00 “security agencies detecting molecular therapeutics” 29:10Improving molecular therapeutic development: 32:00-37:10…
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1 Adeno-associated viral therapies: harnessing the power of HPLC 20:12
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This episode centers on the critical role that high-performance liquid chromatography (HPLC) plays in the production of adeno-associated viral (AAV) therapies, the latest advances in AAV design and the next steps in their development. Filling me in on all things HPLC and AAVs is Bella Neufeld, Director of R&D at Teknova. Bella reveals the essential validation and quality control steps required for the production of successful and safe AAV therapies and the role that both HPLC and liquid chromatography–mass spectrometry (LCMS) play in these steps. We also explore the latest development targets for these therapies, looking at how AAVs can be targeted to specific sites and tissues before descending into the treacherous topic of reproducibility in the field. Listen today to find out the latest tips for best practice when working with HPLC, LCMS the advantages of LCMS over other analytical techniques to assess empty and full capsids and the key principles of AAV production! ContentsIntro: 00:00-01:25Explaining HPLC and its benefits: 01:25-03:30Developing adeno-associated viruses: 03:30-05:25 Chromatography in validation vs production of AAVs: 05:25-06:15Quality control vs hands on time: 06:15-07:35 Challenges in AAV production: 07:35-08:55Examining the level of empty and full capsids: 08:55-10:00The role of LCMS in determining and validating AAV contents: 10:00-11:30 Tips for best practice when using HPLC: 12:30-13:10The future of HPLC: 13:10-14:20Latest advances in AAV development: 14:20-15:50Targeting AAVs: 15:50-17:20Reproducibility issues in AAV development: 17:20-19:35Outro: 19:35-21:10…
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1 Organoids: advancing drug discovery and cancer research 33:07
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As the transition from 2D to 3D cell cultures, or organoids, as the gold standard for modeling basic biology and disease continues, these models are being utilized in ever more intricate and impactful ways. This episode covers the rise of organoids and their advantages over 2D cell cultures, before exploring just how representative these models are becoming and their new applications in drug discovery. To find out all this and more, I speak to Oksana Sirenko, Senior Scientist at Molecular Devices, who works extensively with the company's organoid model development programs. Oksana reveals the potential of these models in cancer therapeutics, driven forward by new patient-derived organoids, and notes the challenges presented by imaging these thicker, 3D models. We also explore the potential discoveries that can be unlocked by combining organoids with organ-on-a-chip models. Contents: What are organoids? 02:10-03:20Functions replicated by organoids? 3:20-04:00What are the advantages of 3D over 2D cell cultures? 04:00-05:40Just how representative are these models? 04:40-07:10 Intestinal organoid formation 07:10-08:50The most exciting application of organoids 08:50-10:10Organoids in drug development 10:10-11:20Methodology of organoids in drug screening studies 11:20-13:30Examples of organoids in cancer drug discovery: 13:30-16:00Limitations of patient-derived and regular organoid models: 16:00-17:50Body-on-a-chip concepts and examples: 17:50-22:00Challenges posed by organoids for drug discovery: 22:00-24:20Techniques improving organoid models and analysis: 24:20-25:50Imaging organoid models: 25:50-26:55Tips for best practice when working with organoids: 26:55-28:20Organoid method development: 28:20-31:10One thing to improve organoid models or analysis for drug screening: 31:10-33:07 To find out more about the research and solutions discussed in this episode visit: www.moleculardevices.com/TechTalk8.…
Automation of ADME: a podcast with Prakash Bhosale by Taylor & Francis Group
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1 Taking an assay from bench to an automated platform: a podcast with Jason Evans 11:18
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In this podcast interview, Jason Evans (Scientist at Q² Solutions; NY, USA) discusses the importance of automation and key advantages of taking an assay from the bench to an automated platform. He also explains the increase in throughput when his lab transitioned to automated assays and the hurdles he faced when automating assays.…
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1 The ethics of whole-genome sequencing: from race and religion to science fiction 27:17
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Welcome to part 2 of this two-part episode on whole-genome sequencing (WGS) in rare disease research and treatment. In part one we discussed the impacts the technology has had on both improving the clinical diagnosis and management of rare diseases and on drug development and basic research studies. This episode goes beyond the technology's capabilities and into the debates surrounding how, when and why they should be used. What needs to change for the technique to be applied equitably? And how does reality stack up against science fiction in terms of people's perceptions of ubiquitous WGS. To do this I speak with David Dimmock, Senior Medical Director of the Rady Children's Institute for Genomic Medicine (CA, USA); Christine Stanley, Chief Director of Clinical Genomics, Variantyx (MA, USA); and Take Ogawa, Vice President of Sales & Marketing at Psomagen (MD, USA). Each of these speakers provides their opinions on the impacts of race, religion and way of life on the application of WGSand how much data is too much data. Contents: Introductions: 00:00-02:20 Gauging public perceptions of routine WGS 02:20-05:40 The role of industry in improving WGS accessibility and capabilities: 05:40-07:35 The appropriate use of pre-symptomatic and predictive risk findings: 08:00-11:15 Debating the discovery of untreatable diseases: 11:15-12:45 The impact of data biases in gene sequencing and the lack of gene references for different populations: 12:45-14:45 The impact of religion and lifestyle on ethical considerations in the use of WGS data: 14:45-16:00 Dealing with unintended findings or results that impact relatives: 16:00-16:45 The value of WGS in pharmacogenomic variant findings and challenging the ACMG59 list: 16:45-19:20 Striking the balance between fully utilizing WGS for all potential findings and ensuring maximum participation for all groups: 19:20-22:45 Filtering data to avoid exclusion: 22:45-24:10 Who owns WGS data? 24:10-25:15 Consent conversations: 25:15-26:30 Final comments: 26:30-27:15…
Love it or hate it, western blotting forms the bedrock of countless studies across numerous disciplines. Explore its history, development and applications in this episode all about the marmite of life science techniques. Guiding me through the hand wringing, hair follicle destroying history and process of western blotting, and hopefully explaining the beauty and potential of the technique - is Kenneth Oh, Senior Project Manager at Bio-Rad Laboratories. Kenneth reveals some of the latest developments in western blotting. Revealing how researchers are now able to validate the success of each stage of a western blot and work with smaller samples, Kenneth provides key tips for ensuring each of your western blots is a triumph. Contents: Intro: 00:00-01:15 History of western blotting: 01:15-3:50 What makes western blotting so tricky? 03:50-05:30 Stepwise optimization: 05:30-06:50 Stain-free western blotting: 06:50-08:50 The different variants of western blotting: 08:50-10:20 The right blot for the right application: 10:20-11:30 The most exciting developments in western bloting:11:30-13:35 New horizons for western blotting: 13:35-15:25 The impact of multiplex-western blotting: 15:25-16:30 Tips for best practice: 16:30-18:10 The future of western blotting: 18:10-20:30 Yearning for automation: 20:30-21:30…
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1 From space to the streets: infectious disease sampling in extreme conditions and extraordinary circumstances 19:21
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In this episode, we take a look at one of the key steps of infectious disease research, sample collection; exploring the challenges associated with the step and the solutions available to improve infectious disease research around the globe. Exploring the trials and tribulations of infectious disease sample collection with me is Stanislav Forman, Product Developer at Zymo Research (CA, USA). Stan explains the extreme environments in which sample collection often occurs; revealing the difficult transit and storage requirements – often involving cold chain logistics – that can derail infectious disease studies early on. Find out how key solutions to these problems have been successfully workshopped with the US military and have even been used in work with NASA to conduct safe sample collection in space. And discover just how successful these solutions can be when faced with the blazing wreckage of a transit vehicle… Contents Introductions: 00:00-01:20Key challenges of sample collection for infectious disease research: 01:20-02:30Additional challenges presented by COVID-19: 02:30-05:00The emergence of at-home testing: 05:00-07:00Established practices for sample collection and introducing DNA/RNA shield: 07:00-09:00Protecting DNA/RNA samples: 09:00-11:00Working with NASA on sample collection and protection in space: 11:00-14:20Protecting samples in a blazing transit van: 14:20-15:25The impact of inactivating and preserving sample collection products on infectious disease research: 15:30-16:30Areas for improvement in sample collection: 16:30-17:30Closing statements: 17:30-19:20…
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1 Revealing the regulome: using multiomic approaches to explore epigenetics and DNA expression 33:28
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Explore the realm of epigenetics and gene expression, discovering the different omic ‘lenses’ used to examine these processes and the power of single-cell studies to reveal provide a comprehensive multiomic view of cells and their biology. This episode features the ever-buoyant Will Greenleaf, Associate Professor of Genetics at Stanford University School of Medicine (CA, USA) and head of the Greenleaf Lab. Will takes us through his lab’s work, detailing his attempts to understand the compacting of DNA into cell nuclei and how this impacts gene expression and epigenetic regulation. Discover the emerging new research focus that Will has coined the “Regulome” – the collection of physical molecular components that control gene expression through factors such as chromatin accessibility. To explore these new frontiers Will often encounters questions with no technologies available to ask them. So what does he do? Invent the technologies he needs! Find out how his lab came to develop the, now widely used, ATAC-Seq and learn of the new tools that he is looking to develop to solve even more perceptive questions. This episode has it all, and also reveals the potential of Will’s single-cell multiomic approaches in cancer research, virology and HIV. Contents: · Introductions: 00:00-02:00 · The exploring the physical genome: 02:00-03:15 · Chromatin accessibility: 03:15-05:15 · Introducing the ‘Regulome’: 05:15-07:15 · The importance of multiomics for the study of gene expression: 07:15-07:55 · DNA methylation and histone organization: 07:55-10:30 · Real-world impact of this research: 10:30-12:30 · Will’s varied career path: 12:30-16:15 · Combining multiomic datasets with computational biology and physics experience: 16:15-18:40 · Single-cell techniques: 18:40-21:50 · Developing ATACC-Seq: 21:50-23:50 · New tools in development: 23:50-27:30 · Insights into the epigenetics of cancer: 27:30-29:35 · Insights into virology and HIV latency: 29:35-32:10 · One wish to help explore the regulome. 32:10-33:00…
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1 Cryopreservation of advanced therapy medicinal products: an interview with Brian Hawkins 10:14
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Cryopreservation of advanced therapy medicinal products: an interview with Brian Hawkins by Taylor & Francis Group
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1 The oncolytic trojan horse: Immune cells, the tumour microenvironment and the invasion of neurons 29:10
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This episode, supported by Bethyl, delves into the realm of the tumor microenvironment (TME), exploring the cells that reside there and how they interact to promote tumor growth and metastasis. Discover how immune cells are attracted and manipulated by tumor cells enabling the cancer to invade neurons where they can then travel throughout the body. Providing an expert's insight into this topic is Moran Amit, Assistant Professor at the MD Anderson Cancer Center (TX, USA). Moran exposes the nebulous interactions in the TME and describes the techniques that he uses to interrogate it, in the hope that by further understanding these interactions we will be able to design more successful, targeted therapies for neurotropic cancers. Contents: · Introductions: 00:00-01:20 · The key cells of the TME: 01:20-02:50 · How cells of the TME support the tumor: 02:50-05:45 · How cancer cells influence and impact immune cells: 05:45-07:25 · The invasion of cancers into the neurons: 07:27-9:45 · Recruitment of neurons to the TME: 09:45-10:55 · The evolution of neuron recruitment to the TME: 10:55-15:00 · Techniques involved in the study of the TME: 15:00-17:00 · Tips for best practice: 17:00-18:00 · Tumors with neural networks: 18:00-20:05 · Clinical impact of research: 20:05-21:50 · The cancers impacted the most by this research: 21:50-23:25 · The impact of neural invasion on patients and neurons: 23:25-25:30 · One thing to improve our understanding of the TME: 25:30-28:00…
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1 Whole-genome-sequencing: navigating the "Diagnostic Odyssey" in rare disease research 39:28
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Rare diseases present a series of challenges on numerous fronts. From simply deciphering what a patient is affected by to considering how best to manage a rare condition with limited pre-existing medications available for a rare disease, each new development or breakthrough brings clinicians to a new question. While these conditions are, in isolation, rare; in total rare diseases impact 25-30million people in the USA alone. Supported by Psomagen and featuring four fascinating guests from across the spectrum of topics and focuses in the rare disease space, this episode explores the updates to whole-genome sequencing technology that have improved the "Diagnostic Oddysey" experienced by some patients, enabled more accurate drug development and provides insight into the diagnosis and characterization of undefined rare diseases, before exploring the clinical impact whole-genome sequencing can have on clinical outcomes. This episode features insight from four field-leading experts in rare disease research, whole-genome sequencing technologies and clinical practice for patients with rare diseases, including: Alan Beggs: Director of The Manton Center for Orphan Disease Research, Sir Edwin and Lady Manton Professor of Pediatrics at Harvard medical school Christine Stanley: Chief Director of Clinical Genomics at VariantyxDavid Dimmock: Senior Medical Director of Rady Children's Institute for Genomic MedicineTake Ogawa: Vice President, Sales & Marketing at Psomagen Contents Introduction: 00:00-02:30Defining the Diagnostic Oddysey: 02:30-06:20Rare disease discovery: 06:20-09:20Techniques involved in Rare disease discovery: 09:20-12:05Whole-genome sequencing updates impacting clinical diagnosis: 12:05-18:30Currently required improvements in techniques for rare disease research and management: 18:30-24:10Explaining long-read and short-read sequencing: 24:10-26:00Developing therapeutics for rare diseases: 26:00-30:00Whole-genome sequencing in animal model validation: 30:00-31:20Improving access to whole-genome available to researchers and clinicians: 31:20-33:40Whole-genome sequencing improving management and outcomes for rare diseases: 33:40-38:27…
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1 In the Zone: phosphorylated biomarkers 16:20
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In the Zone: phosphorylated biomarkers by Taylor & Francis Group
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1 Environmental sequencing for COVID-19 28:46
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In this episode, we explore the often overlooked technique of environmental sequencing for infectious diseases and how it is being applied to the response to COVID-19. Taking the state of California and a case study of this application, I speak to Micheal Lisek, Project Manager Environmental Microbiomics at Zymo Research Corp. Micheal outlines the basis of environmental sequencing, predominantly the screening of wastewater for signs of viral RNA, the key techniques involved in the process and challenges associated with it. Micheal also explains the key pieces of information that can be obtained by environmental sequencing and the benefits of the approach vs exclusively using clinical testing. Micheal highlights the value of environmental sequencing for monitoring variants, understanding transmission patterns and identifying outbreaks, providing examples of the information provided to the public health officials in California that were used to successfully manage rising infections and limit the impact of a surge in cases. Contents Introduction: 00:00-01:58Introducing environmental surveillance for infectious diseases: 01:58-03:30COVID-19 response vs previous environmental sequencing work and how it has prepared people for COVID-19: 03:30-06:18The benefits of wastewater screening over clinical testing: 06:18-07:10Challenges of wastewater testing: 07:10-09:10Monitoring for SARS-CoV-2 variants: 09:10-10:55Understanding transmission patterns: 10:55-12:00Identifying signs of viral vaccine escape and tracing transmission: 12:00-14:50PCR vs NGS techniques for environmental sequencing: 14:50-16:05Tips for best practice when conducting environmental sequencing: 16:05-17:50Practical results of environmental sequencing data on outbreak suppression: 17:50-20:40Investigating mutation rates in the environmental samples: 20:40-23:20Emerging techniques in wastewater surveillance: 23:20-25:10Developing standard protocols and structure for wastewater surveillance: 25:10-28:20…
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1 Implementing sensors into cell culture systems: an interview with Abbie Underhill 11:21
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In this podcast, Tristan Free – Digital Editor at RegMedNet – speaks to Abbie Underhill (Scientific Bioprocessing) about the role that sensors have in regenerative medicine, including the biggest challenges behind implementing sensors in cell culture systems.
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1 How PCR has prevailed during the COVID-19 pandemic 30:03
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In Part 3 of our COVID-19 diagnostics and detection miniseries, supported by Roche, we explore the contribution PCR has made towards diagnosis during the pandemic. Providing me with an insight into the world of PCR diagnostics is Tyler Miller, Clinical Pathology Resident and Research Fellow at Massachusetts General Hospital, where he was instrumental in setting up the testing regimen for the Hospital. Ty details the attributes of PCR that lead to it becoming the gold standard for diagnostic tests, before explaining the variance in clinical detection rate during a patient's disease course and how this variation correlates with the infectivity of the patient. Ty also delves into the variety of sample collection methods available, how each of these methods compares in terms of sensitivity and their ability to be integrated into exciting novel PCR techniques. We also look at the work of the Broad Institute in establishing a mass testing effort that involved automation, workflow optimization and hundreds of new staff, which lead to the delivery of 100,000 tests a day, 5% of the USA's COVID-19 testing at the time. All of this rapid work has led to dramatic changes in the PCR technique and process. These changes are perhaps exemplified by PCR testing without RNA extraction and purification, which Ty explains was partly developed due to the limited supplies available to researchers at the beginning of the pandemic. Contents Introduction: 00:00-02:00Why PCR tests are so valuable: 02:00-03:10Changes in clinical detection rate and infectivity: 03:10-8:00Infectivity and exceptions to the rule: 08:00-09:00Sample collection methods, sensitivity and encouraging testing uptake 09:00-17:00The Broad Institute's mass testing regimen: 17:00-21:00Developments during the pandemic and no RNA extraction PCR: 21:00-27:00Dreams for improving COVID-19 testing: 27:00- 30:00 This episode was supported by Roche sequencing and life science. Follow these links to find out more about the LightCycler 480 System and LightCycler 96 System.…
Returning to the contentious topic of the Reproducibility crisis – the inability of many study results to be replicated by different research groups or labs – this episode, supported by Zymo Research, zeros in on the topic within the field of microbiomics. Speaking to me about the topic is Raul Cano, Chief Scientific Officer at the BioCollective. Raul discusses the crisis and explains why it is prevalent in the emerging field of microbiomics before taking a look at the key role that microbiomics is beginning to play in the field of diagnostics, and how – if we make changes now – lasting improvements can be made in the field. Raul also lays out the three key areas that are holding back reproducibility in microbiomics and explains the actions that can be taken to improve the situation. Contents: Introduction: 00:00-02:00Explaining the reproducibility crisis: 02:00-02:55How microbiomics compare to other fields in terms of reproducibility: 02:55-04:05Challenges in reproducibility specific to microbiomics: 04:05-05:45The importance of reproducibility in microbiomics: 05:45-07:00Microbiomics in diagnostics: 07:00-10:00 Three key causes of poor reproducibility in microbiomics: 10:00-11:30Is detailed documentation improving in the life sciences? 11:30- 13:00Actions that can improve reproducibility: 13:00-14:15Fecal references: 14:15-16:05The realities of preparing fecal references: 16:05-18:1 5The reception to the new fecal references: 18:15-20:35References vs Standards: 20:35-22:15Standardizing microbiomics data: 22:15-23:20Assessing reproducibility in previous studies: 23:20-25:15Dreams for reproducibility in microbiomics: 25:15-26:55 This episode is supported by Zymo Research. If you would like more information about the fecal reference mentioned in the podcast, you can visit the product page here.…
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1 Spotlight: precision medicine and companion diagnostics 10:35
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Spotlight: precision medicine and companion diagnostics by Taylor & Francis Group
Virtual audits: limitations and future trends by Taylor & Francis Group
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1 How can we achieve gender equality in STEM? 40:10
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This International Women’s Day takeover episode, with special guest host BioTechniques’ Senior Digital Editor Abi Sawyer, takes a look at the results of Future Science Group’s (London, UK) survey for the scientific community on gender equality and parity in STEM. Abi’s guests on this episode are the Vice President of Epidemiology and Clinical Evidence at IQVIA (NC, USA), Dr Christina Mack; the Executive Director for the Pharmaceutical Research Computing Center at the University of Maryland School of Pharmacy (MD, USA), Dr Ebere Onukwugha; a Lecturer, Science Communicator and Author based in Cardiff (UK), Dr Emma Yhnell; and the Director of the Neuroscience Center Microscopy Core at the University of North Carolina–Chapel Hill (NC, USA), Dr Michelle Itano. They discuss the results of Future Science Group’s survey, share their own experiences of gender inequality as well as situations where they’ve felt supported, and outline how the STEM community can push further towards gender equality and parity. Contents: Introduction: 00:00 – 1:47 Gender inequality, experiences and impact on STEM careers: 1:48 – 10:41 Underlying reasons for gender disparity in STEM: 10:42 – 17:51 Promoting gender equality in the STEM work environment: 17:52 – 30:34 Looking forward: representation and mentoring: 30:35 – 39:32 Conclusions: 39:33 – 40:10…
3DMedNet International Women's Day: an interview with Lee-Bath Nelson by Taylor & Francis Group
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1 COG-UK: sequencing SARS-CoV-2 and detecting the novel variant B.1.1.7 34:41
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This episode takes a look at the role the COVID-19 Genomics UK Consortium (COG-UK) has played sequencing SARS-CoV-2 and surveying for COVID-19. To do this I speak to two key members of the consortium; Steve Paterson, Professor of genetics at the University of Liverpool and lead for the wastewater working group of COG-UK; and Josh Quick, Future Leaders Fellow at the University of Birmingham and lead at the COG-UK Sequencing working group. Steve discusses some of the techniques required to detect SARS-CoV-2 in wastewater, the challenges that such a vibrant sample can present, and gives his account of the part that wastewater surveillance played in the management of the new variant B.1.1.7. Josh provides us with further insight into the technologies used to sequence SARS-CoV-2 and explains how he designed the ARTIC protocol for sequencing the virus and why it came to be so widely used. Josh also explains the bizarre act of serendipity that aided in the discovery of the B.1.1.7 variant.…
In the Zone: how do we decide a vaccine is effective? by Taylor & Francis Group
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1 COVID-19 diagnostics: which should you choose? 21:14
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RT-PCR, antigen and antibody testing; each has its niche and each has a flaw. But what are the key differences between each of these tests and when what are the key things to consider when selecting the appropriate COVID-19 diagnostic for you? In this podcast, supported by Zymo Research, I speak to Senior Scientist in assay development Paolo Piati in order to answer this question. Whether you are a concerned citizen looking for the best test to take to inform your behavior, or a researcher aiming to understand which testing systems you need to set up in your lab – this episode should have the answers for you. In addition to these key points, Paolo addresses the importance of testing during the rollout of vaccine programs and issues of false negatives in diagnostics, examining what, if anything, can be done to improve the accuracy of testing programs.…
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1 The importance of bacteriophage collections 26:08
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In this podcast, Juandem Agendia and Sarah Alexander (NCTC, UK) discuss bacteriophages, the function of the NCTC and the importance of depositing bacteriophages in the NCTC collection to improve accessibility and reproducibility in science.
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1 OCTalks: Comprehensive Genomic Profiling 19:01
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OCTalks: Comprehensive Genomic Profiling by Taylor & Francis Group
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1 OCTalks: the ethics of COVID-19 and cancer 10:41
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OCTalks: the ethics of COVID-19 and cancer by Taylor & Francis Group
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1 OCTalks: targeting FGFR2 in advanced cholangiocarcinoma 10:28
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OCTalks: targeting FGFR2 in advanced cholangiocarcinoma by Taylor & Francis Group
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1 The importance of data management: an interview with Xiaonan Tang 10:32
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The importance of data management: an interview with Xiaonan Tang by Taylor & Francis Group
Brendan Griffin (University College Cork, Ireland)discusses his work in early drug development in this interview with Bioanalysis Zone. Brendan talks about the most common challenges faced as well as key trends in the field of developability. For more content, visit www.bioanalysis-zone.com
In this Technique Blast – a new series that will provide short informative discussions about a specific technique – Digital Editor Tristan Free speaks to Joseph Pesek about high-performance liquid-chromatography and chromatographic separation technologies.
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1 3DMedTALKS | The additive response to COVID-19 49:50
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In the face of the COVID-19 pandemic, innovators are finding novel ways to apply 3D-printed solutions to assist the international response to coronavirus. From meeting PPE demands, to designing novel approaches to unique challenges, the additive community has not been short of opportunity for collaboration and corroboration with multidisciplinary teams exploring options for aiding crises from local to international capacities. In this podcast, in collaboration with Women in 3D Printing, we invited a panel of experts to take a look at how the additive community has responded to coronavirus, where the limitations lie in integrating 3D-printed devices into a health system under pressure as well as what can be done by the wider 3D printing community to help overcome critical challenges posed by COVID-19. Meet the experts > Maxine Chan Director of Med Supply Drive UK, NHS Obstetrician and Gynaecologist, PhD Researcher in Bioengineering and Regenerative Medicine, Imperial College London (UK) Maxine Chan is an NHS Doctor and Researcher in London with a research interest in innovative bioengineering solutions to treating female reproductive health issues. Chan is also the Director of Med Supply Drive UK (London, UK), an organization of volunteer medics and non-medics who are protecting frontline healthcare workers during the COVID-19 crisis by providing solutions to PPE shortages. > Brigette De Vet-Veithen VP and General Manager, Materialise Medical Brigitte de Vet-Veithen has been the Vice President of Materialise Medical since June 2016, as a representative of De Vet Management bvba. De Vet-Veithen has almost 20 years of experience in the healthcare and life sciences sector. She has worked in various management roles for Johnson & Johnson (NJ, USA), ultimately serving as Vice President for the EMEA region of Cordis Neurovascular and General Manager of Cordis (CA, USA) in Germany. Before joining Materialise (Leuven, Belgium), she held various leadership roles as a representative of De Vet Management bvba including the role of Chief Executive Officer of Acertys group (Aartselaar, Belgium), a provider of medical devices, software, services and supplies to hospitals and medical professionals. De Vet-Veithen holds a Master of Business Administration with a Major in Engineering from HEC Liege (Belgium) and an MBA from INSEAD (Fontainebleau, France). > Kate Hammer Co-Founding Committee Member, SHIELD Collaborative Kate Hammer is an innovation specialist ranging across architecture/engineering, biotechnology, chemical, FMCG, healthcare, information, public health and technology sectors, often working with scientists of all descriptions to realize demanding projects, often at speed. > Kadine James Co-Founder, SHIELD Collaborative Learner, maker, innovator and 3D technology enthusiast, Kadine James is the Creative Tech Lead at Hobs 3D (London, UK) and is a prominent UK tech evangelist. Combining 7+ years’ experience in 3D printing, virtual reality, augmented reality and mixed reality, James is driven by big ideas, a global mind-set and empowering the use 3D printing in art and architecture. She sets out to create a platform for 3D printing VR/AR/MR learning opportunities for young people to get involved with 3DTech in central London, at Hobs 3D’s hub and incubator for thought leadership. James is UK Lead of Women in 3D Printing, a global platform which inspires, encourages and supports women working with rapid prototyping technologies – and is one of the first Industry Champions for the Creative Industries Policy & Evidence Centre network (led by Nesta). James is listed as one of the top 100 most influential women in UK Tech.…
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1 Integrating patient-relevant outcomes into clinical practice: an interview with Donna Messner 27:58
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Integrating patient-relevant outcomes into clinical practice: an interview with Donna Messner by Taylor & Francis Group
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1 NCTalks with Gavin Giovannoni: advancements in the treatment landscape for multiple sclerosis 17:42
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NCTalks with Gavin Giovannoni: advancements in the treatment landscape for multiple sclerosis by Taylor & Francis Group
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1 Comparing real-world and randomized controlled trial data: an interview with Michael Moran 18:26
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Comparing real-world and randomized controlled trial data: an interview with Michael Moran by Taylor & Francis Group
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1 Talking Techniques | UV LEDs for disinfection and potential protection from SARS-CoV-2 30:28
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BioTechniques Digital Editor Tristan Free, speaks to Application Scientist Theresa Thompson and Strategy Director Jay Pasquantonio (both Phoseon Technology) about their UV LEDs. We cover the best wavelengths for disrupting nucleotides and proteins, the challenges associated with some of these wavelengths, and the promising potential of UV LEDs to disinfect inorganic surfaces of SARS-CoV-2. Theresa and Jay also detail the safety procedures required while using this technology and deliver a clear warning against the irradiation of organic matter with UV LEDs.…
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1 Estimating the opportunity–cost-based cost–effectiveness threshold: a panel discussion 18:12
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Estimating the opportunity–cost-based cost–effectiveness threshold: a panel discussion by Taylor & Francis Group
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1 Talking Techniques: Donald Ingber on COVID-19, organ-on-a-chip technology and the Wyss Institute 33:10
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In the first installment of this two-part episode with Donald Ingber, Founding Director of the Wyss Institute (MA, USA), we discuss his invention of organ-on-a-chip technology, how he is utilizing them in the fight against COVID-19 and the Wyss Institute's response to the pandemic. Donald touches on the performance of the much-discussed chloroquine in his lung-on-a-chip models. We also discuss the pros and cons of organ-on-a-chip technology verses organoids, exploring their use in combination, before Donald examines the possible replacement of animal models with organ-on-a-chip technology.…
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1 Talking Techniques | Microbiome studies; big data analysis, LCMS and at-home fecal sample collection 31:15
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In this episode BioTechniques Digital Editor, Tristan Free speaks to Annie Evans, Director of R&D and David Foster, Vice President of Data and Informatics at Metabolon about the latest developments in microbiome studies, including big data analysis, LCMS, alpha diversity and at-home fecal sample collection.…
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1 Talking Techniques | Paul Brennan: repairing nerve damage and addressing Alzheimer's 25:33
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